粳稻杂种后代胚乳可溶性淀粉合成酶及同工型基因表达特性分析

doi:10.3969/j.issn.1001-7216.2014.01.004

摘要/Abstract

摘要： 以籽粒直链淀粉含量为选择指标，从东农423×藤系180 F2代起连续定向选择培育成的籽粒直链淀粉含量有显著差异的F10后代东农1006（直链淀粉含量为18.82%）、东农1012（直链淀粉含量为7.70%），同样，从系选1号×通769后代中选出东农1001（直链淀粉含量为17.51%）、东农1024（直链淀粉含量为7.40%）比较分析了灌浆过程中籽粒直链淀粉含量和可溶性淀粉合成酶活性及同工型基因表达量等变化特征。结果表明，灌浆不同时期籽粒直链淀粉含量低的后代及亲本直链淀粉量始终低于直链淀粉含量高的后代及亲本；成熟期籽粒直链淀粉含量低的亲本和后代籽粒支链淀粉含量均显著高于籽粒直链淀粉含量高的亲本和后代，抽穗后10 d、17 d、24 d的籽粒直链淀粉含量与支链淀粉含量间均呈负相关，但相关未达显著水平；抽穗后10 d、17 d、24 d的可溶性淀粉合成酶活性与籽粒直链淀粉含量的相关系数分别为0.8115**， -0.7554*， -0.5957，与支链淀粉含量的相关系数分别为-0.0694， 0.5453， -0.0207；在灌浆过程中亲本及后代的胚乳OsSSSⅠ、 OsSSSⅡ1、 OsSSSⅡ3、 OsSSSⅢ1和 OsSSSⅢ2基因随籽粒灌浆进程其mRNA转录表达量逐渐增加，达到峰值后又逐渐下降，呈单峰曲线变化；而OsSSSⅢ2和OsSSSⅣ2基因mRNA转录表达量在灌浆各时期都比其他基因大；灌浆不同时期直链淀粉含量高的后代与高亲相比、直链淀粉含量低的后代与低亲相比，既有转录表达量增加的基因，也有降低的基因；可溶性淀粉合成酶活性与OsSSSⅠ、OsSSSⅡ3、OsSSSⅢ2的mRNA转录表达量正相关，相关达显著水平；与OsSSSⅡ1、OsSSSⅣ2的mRNA转录表达量正相关，但相关未达显著水平，与OsSSSⅢ1的mRNA转录表达量呈负相关，但相关未达显著水平。

关键词: 粳稻, 杂种后代, 胚乳, 可溶性淀粉合成酶基因, 表达分析

Abstract: Using amylose content as the selection indicator, we chose the progenies with significantly different amylose content in grains by continuous and unidirectional orthoselection from F2 to F10 generations, compared the progeny of Dongnong 1006（Its grain amylose content was 18.82%）and Dongnong 1012（7.70%）with their parent Dongnong 423（18.33%）and Toukei 180（11.05%）, respectively. And we also compared the progeny of Dongnong 1006（18.82%）and Dongnong 1012（7.70%）with their parent Xixuan 1（18.74%）and Tong 769（17.05%）. Moreover, all groups above were chosen to analyze amylose accumulation and soluble starch synthase(SSS) activity and the expression levels of isoform genes during the grain filling. The results showed that the accumulated amount of amylose in parents and hybrid progeny with high amylose contents were always higher than that in the low amylose content progenies in different periods of grain filling, but for the amylopectin content, there were opposite results in the maturity stage. There were no significant negative correlations between the grain amylose content and amylopectin content 10, 17, 24 days after heading, and the correlation coefficients between SSS activity and the grain amylose content were 0.8115**, -07554*, -0.5957, respectively. In addition, the correlation coefficients between SSS activity and amylopectin content were -0.0694, 0.5453, -0.0207, respectively. The expression levels of the OsSSSⅠ,OsSSSⅡ1, OsSSSⅡ3, OsSSSⅢ1 and OsSSSⅢ2genes in endosperm of parents and hybrid progenies gradually peaked and then gradually declined, following a single peak curve along with the process of grain filling, while the expression levels of the OsSSSⅣ2 gene just showed a downdraft. However, the expression levels of the OsSSSⅢ2 and OsSSSⅣ2 genes were higher than others in various periods of grain filling. Compared with their parents, hybrid progeny with high amylose content have the expression of increased genes and reducing genes during the different period of filling, as well as the hybrid progeny with low amylose content compared with their parents. Moreover, there was significantly positive correlation between SSS activity and the expression levels of the OsSSSⅠ,OsSSSⅡ3,OsSSSⅢ2,and no significant positive correlation between SSS activity and the expression of the OsSSSⅡ1 and OsSSSⅣ2, and no significant negative correlation between SSS activity and the expression of the OsSSSⅢ1.

Key words: japonica, hybrid progeny, endosperm, soluble starch synthase genes, expression analysis

中图分类号:

Q786
S511.01

曲莹,金正勋*,刘海英,徐振华,朱立楠,郑冠龙,朱方旭,张忠臣. 粳稻杂种后代胚乳可溶性淀粉合成酶及同工型基因表达特性分析[J]. 中国水稻科学, 2014, 28(1): 23-31.

QU Ying, JIN Zhengxun*, LIU Haiying, XU Zhenhua, ZHU Linan, ZHENG Guanlong, ZHU Fangxu, . Analysis of Expression Characters of Soluble Starch Synthase and Isoform Genes Involved in japonica Hybrid Progeny[J]. Chinese Journal of Rice Science, 2014, 28(1): 23-31.

参考文献

\[1\]Reddy K R, Ali S Z, Bhattacharya K R. The fine structure of rice starch amylopectin and its relation to the texture of cooked rice. Carbohydr Polym, 1993, 22(4): 267275.

\[2\]程方民, 钟连进, 孙宗修. 灌浆结实期温度对早籼水稻籽粒淀粉合成代谢的影响. 中国农业科学, 2003, 36(5): 492501.

\[3\]Keeling P L, Bacon P J, Holt D C. Elevated temperature reduces starch deposition in wheat endosperm by reducing the activity of soluble starch synthase. Planta, 1993, 191(3): 342348.

\[4\]李木英, 石庆华, 胡志红, 等. 高温胁迫对不同早稻品种胚乳淀粉合成酶类活性的影响. 中国农业科学, 2007, 40(8):16221629.

\[5\]韦克苏, 张其芳, 程方民, 等. 花后高温下水稻可溶性淀粉合成酶同工型基因的表达模式. 作物学报, 2009, 35(1): 1824.

\[6\]吕冰, 郭志刚, 梁建生. 水稻胚乳中淀粉合成相关酶活性的变化对支链淀粉精细结构的影响. 中国科学C辑:生命科学, 2008, 38(8): 766773.

\[7\]沈鹏, 金正勋, 罗秋香, 等. 水稻灌浆过程中籽粒淀粉合成关键酶活性与蒸煮食味品质的关系. 中国水稻科学, 2006, 20(1): 5864.

\[8\]Zhao B H, Zhang W J. Changes in activities of the key enzymes related to starch synthesis in rice grains during grain filling and their relationships with the filling rate and cooking quality. Agric Sci China, 2005, 4(1): 2633.

\[9\]Manish K, Pandey N, Rani S. Different isoforms of starchsynthesizing enzymes controlling amylose and amylopectin content in rice (Oryza sativa L.). Biotechnol Adv, 2012, 30(6): 16971706.

\[10\]程方民, 蒋德安, 吴平, 等. 早稻籽粒灌浆过程中淀粉合成酶的变化及温度效应特征. 作物学报, 2001, 27(2): 201206.

\[11\]何照范. 粮油籽粒品质及其分析技术. 北京: 中国农业出版社, 1985: 137139.

\[12\]赵步洪, 张文杰, 常二华, 等. 水稻灌浆期籽粒中淀粉合成关键酶的活性变化及其与灌浆速率和蒸煮品质的关系. 中国农业科学, 2004, 37(8): 11231129.

\[13\]董明辉, 赵步洪, 吴翔宙, 等. 水稻结实期不同粒位籽粒相关内源激素含量和关键酶活性的差异及其与品质的关系. 中国农业科学, 2008, 41(2): 370380.

\[14 \]Hirose T, Terao T. A comprehensive expression analysis of the starch synthase gene family in rice(Oryza sativa L.). Planta, 2004, 220(1): 916.

\[15\]Denyer K, Smith A M. The purification and characterization of the two forms of soluble starch synthase from developing pea embryos. Planta, 1992, 186(4): 609627.

\[16\]Marshall J, Sidebottom C, Debet M. Identification of the major starch synthase in the soluble fraction of potato tubers. Plant Cell, 1996, 8(7): 11211135.

\[17\]Ohdan T, Francisco P B Jr， Sawada T, et al. Expression profiling of genes involved in starch synthesis in sink and source organs of rice. J Exp Bot, 2005, 56(422): 32293224.

\[18\]Baba T, Nishihara M, Mizuno K, et al. Identification, cDNA cloning, and gene expression of soluble starch synthase in rice (Oryza sativa L.) immature seeds. Plant Physiol, 1993, 103(2): 565573.

\[19\]朱军, 刘庆昌, 张天真, 等. 遗传学. 3版. 北京: 中国农业出版社, 2002: 312314.

\[20\]金正勋, 王露露, 刘海英, 等. 粳稻杂种后代胚乳淀粉分支酶基因表达特性分析. 东北农业大学学报, 2013, 44(1): 1823.

\[21\]刘海英, 李晓光, 刘洪亮, 等. 水稻杂种后代籽粒直链淀粉含量选择对品质和产量性状的影响. 中国水稻科学, 2009, 23(1): 107110.

\[22\]Vandeputte G E, Delcour J A. From sucrose to starch granule to starch physical behavior: A focus on rice starch. Carbohydr Polymer, 2004, 58(3): 245266.

\[23\]Nakamura Y. Towards a better understanding of the metabolic system for amylopeetin biosynthesis in plantsrice endosperm as a model tissue. Plant Cell Physiol, 2002, 43(7): 718–725.

\[24\]Commuri P D, Keeling P L. Chainlength specificities of maize starch synthase I enzyme: Studies of glucan affinity and catalytic properties. Planta, 2001, 25(5): 475486.

\[25\]Martha G, James Y, Kay D. Starch synthesis in the cereal endosperm. Curr Opin Plant Biol, 2003, 6(3): 215222.

\[26\]朱昌兰, 翟虎渠, 万建民. 稻米食味品质的遗传和分子生物学基础研究. 江西农业大学学报: 自然科学版, 2002, 24(4): 454

[1]	黄亚茹, 徐鹏, 王乐乐, 贺一哲, 王辉, 柯健, 何海兵, 武立权, 尤翠翠. 外源海藻糖对粳稻品系W1844籽粒灌浆特性及产量形成的影响[J]. 中国水稻科学, 2023, 37(4): 379-391.
[2]	马兆惠, 石一涵, 程海涛, 宋文雯, 路连吉, 刘仁广, 吕文彦. 水稻种子胚形态与胚乳组成对稻米留胚特性的影响[J]. 中国水稻科学, 2023, 37(3): 265-275.
[3]	王雨, 孙全翌, 杜海波, 许志文, 吴科霆, 尹力, 冯志明, 胡珂鸣, 陈宗祥, 左示敏. 利用抗稻瘟病基因Pigm和抗纹枯病数量性状基因qSB-9^TQ、qSB-11^HJX改良南粳9108的抗性[J]. 中国水稻科学, 2023, 37(2): 125-132.
[4]	姚姝, 赵春芳, 陈涛, 路凯, 周丽慧, 赵凌, 朱镇, 赵庆勇, 梁文化, 赫磊, 王才林, 张亚东. 低谷蛋白半糯型粳稻营养品质与蒸煮食味品质特征分析[J]. 中国水稻科学, 2023, 37(2): 178-188.
[5]	裴峰, 王广达, 高鹏, 冯志明, 胡珂鸣, 陈宗祥, 陈红旗, 崔傲, 左示敏. 敲除OsNramp5基因创制低镉优质粳稻新材料的应用评价[J]. 中国水稻科学, 2023, 37(1): 16-28.
[6]	陈涛, 赵庆勇, 朱镇, 赵凌, 姚姝, 周丽慧, 赵春芳, 张亚东, 王才林. 利用分子标记辅助选择培育优良食味、低谷蛋白香粳稻新品系[J]. 中国水稻科学, 2023, 37(1): 55-65.
[7]	王永祥, 燕海刚, 徐含聪, 傅玉双, 单壮壮, 胡晓晴, 张文伟, 江玲. OsESV1基因对水稻淀粉合成的影响[J]. 中国水稻科学, 2022, 36(2): 139-149.
[8]	王才林, 张亚东, 陈涛, 朱镇, 赵庆勇, 姚姝, 赵凌, 赵春芳, 周丽慧, 魏晓东, 路凯, 梁文化. 姊妹系间杂交快速培育优良食味半糯粳稻新品种的育种效果[J]. 中国水稻科学, 2021, 35(5): 455-465.
[9]	王孟佳, 殷敏, 褚光, 刘元辉, 徐春梅, 章秀福, 王丹英, 陈松. 长江中下游双季晚粳稻产量、生育时期及温光资源配置的生态性差异[J]. 中国水稻科学, 2021, 35(5): 475-486.
[10]	张娟, 牛百晓, 鄂志国, 陈忱. 水稻胚乳发育遗传调控的研究进展[J]. 中国水稻科学, 2021, 35(4): 326-341.
[11]	王才林, 张亚东, 陈涛, 朱镇, 赵庆勇, 赵春芳, 姚姝, 周丽慧, 赵凌, 魏晓东, 路凯, 梁文化. 地点和播期对半糯粳稻食味品质的影响[J]. 中国水稻科学, 2021, 35(4): 373-382.
[12]	张庆, 胡雅杰, 郭保卫, 张洪程, 徐晓杰, 徐玉峰, 朱邦辉, 徐洁芬, 钮中一, 凃荣文. 太湖地区优良食味高产软米粳稻品种特征研究[J]. 中国水稻科学, 2021, 35(3): 279-290.
[13]	路凯, 陈涛, 姚姝, 梁文化, 魏晓东, 张亚东, 王才林. 盐胁迫下四个水稻类受体蛋白激酶的功能分析[J]. 中国水稻科学, 2021, 35(2): 103-111.
[14]	杨宇尘, 夏原野, 闫志强, 汪磊, 闫秉春, 王镜博, 王祎玮, 栾金华, 徐海. 抽穗开花期喷施茉莉酸甲酯对不同生态区粳稻花时和株型的影响[J]. 中国水稻科学, 2021, 35(1): 69-77.
[15]	唐小涵, 刘世家, 刘喜, 田云录, 王云龙, 滕烜, 段二超, 张元燕, 江玲, 张文伟, 王益华, 万建民. 色氨酰-tRNA合成酶基因WRS1调控水稻种子发育[J]. 中国水稻科学, 2020, 34(5): 383-396.